Tailoring a robust and recyclable nanobiocatalyst by immobilization of Pseudomonas fluorescens lipase on carbon nanofiber and its application in synthesis of enantiopure carboetomidate analogue.
Int J Biol Macromol
; 133: 1299-1310, 2019 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-30940586
ABSTRACT
Pseudomonas fluorescens lipase (PFL) was covalently immobilized on carbon nanofiber (CNF) using 1ethyl3[3dimethylaminopropyl] carbodiimide (EDC)/Nhydroxysuccinimide (NHS). Surface functionalization of carbon nanofiber augments dispersibility as well as efficiency of covalent immobilization. Crucial parameters for immobilization such as pH, enzyme-support ratio, reaction time and mixing rate were optimized using one factor at a time (OFAT) approach. The nanobiocatalyst prepared under optimized conditions demonstrated a ten-fold increase in enzyme activity and the advantage of high thermal stability (up to 85⯰C) along with 10â¯cycles of reusability. Subsequently practical application of the nanobiocatalyst was explored in the kinetic resolution of racemic 1phenylethanol into (S)1phenylethanol [Câ¯=â¯49.1%, eepâ¯=â¯99.5%, eesâ¯=â¯98.5% and E valueâ¯=â¯151.4] followed by Mitsunobu reaction with a substituted pyrrole, giving an enantiopure (R)-carboetomidate analogue (yieldâ¯=â¯83%).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrroles
/
Pseudomonas fluorescens
/
Carbon
/
Enzymes, Immobilized
/
Nanofibers
/
Lipase
Language:
En
Journal:
Int J Biol Macromol
Year:
2019
Type:
Article
Affiliation country:
India