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A high-affinity peptide substrate for G protein-coupled receptor kinase 2 (GRK2).
Asai, Daisuke; Murata, Masaharu; Toita, Riki; Kawano, Takahito; Nakashima, Hideki; Kang, Jeong-Hun.
Affiliation
  • Asai D; Department of Microbiology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki, 216-8511, Japan. asai@marianna-u.ac.jp.
  • Murata M; Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
  • Toita R; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
  • Kawano T; AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Nakashima H; Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
  • Kang JH; Department of Microbiology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki, 216-8511, Japan.
Amino Acids ; 51(6): 973-976, 2019 Jun.
Article in En | MEDLINE | ID: mdl-31004228
ABSTRACT
We synthesized a previously identified ß-tubulin-derived G protein-coupled receptor kinase 2 (GKR2) peptide (GR-11-1; DEMEFTEAESNMN) and its amino-terminal extension (GR-11-1-N; GEGMDEMEFTEAESNMN) and carboxyl-terminal extension (GR-11-1-C; DEMEFTEAESNMNDLVSEYQ) peptides with the aim of finding a high-affinity peptide substrate for GRK2. GR-11-1-C showed high affinity for GRK2, but very low affinity for GKR5. Its specificity and sensitivity for GKR2 were greater than those of GR-11-1 and GR-11-1-N. These findings should be useful in designing tools for probing GKR2-mediated intracellular signaling pathways, as well as GRK2-specific drugs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / G-Protein-Coupled Receptor Kinase 2 / G-Protein-Coupled Receptor Kinase 5 Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Amino Acids Journal subject: BIOQUIMICA Year: 2019 Type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / G-Protein-Coupled Receptor Kinase 2 / G-Protein-Coupled Receptor Kinase 5 Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Amino Acids Journal subject: BIOQUIMICA Year: 2019 Type: Article Affiliation country: Japan