Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Nat Methods
; 16(7): 587-594, 2019 07.
Article
in En
| MEDLINE
| ID: mdl-31249407
ABSTRACT
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Proteins
/
Benchmarking
Type of study:
Guideline
/
Prognostic_studies
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2019
Type:
Article
Affiliation country:
United States