Comprehensive model for X-ray-induced damage in protein crystallography.
J Synchrotron Radiat
; 26(Pt 4): 945-957, 2019 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-31274416
ABSTRACT
Acquisition of X-ray crystallographic data is always accompanied by structural degradation owing to the absorption of energy. The application of high-fluency X-ray sources to large biomolecules has increased the importance of finding ways to curtail the onset of X-ray-induced damage. A significant effort has been under way with the aim of identifying strategies for protecting protein structure. A comprehensive model is presented that has the potential to explain, both qualitatively and quantitatively, the structural changes induced in crystalline protein at â¼100â
K. The first step is to consider the qualitative question what are the radiation-induced intermediates and expected end products? The aim of this paper is to assist in optimizing these strategies through a fundamental understanding of radiation physics and chemistry, with additional insight provided by theoretical calculations performed on the many schemes presented.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
X-Rays
/
Proteins
/
Models, Molecular
/
Crystallography, X-Ray
Type of study:
Prognostic_studies
/
Qualitative_research
Language:
En
Journal:
J Synchrotron Radiat
Journal subject:
RADIOLOGIA
Year:
2019
Type:
Article
Affiliation country:
United States