Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno-associated virus production by fluorescence spectroscopy.

Pais, Daniel A M; Portela, Rui M C; Carrondo, Manuel J T; Isidro, Inês A; Alves, Paula M

Pais, Daniel A M; Portela, Rui M C; Carrondo, Manuel J T; Isidro, Inês A; Alves, Paula M.

Affiliation

Pais DAM; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
Portela RMC; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Carrondo MJT; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
Isidro IA; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
Alves PM; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.

Biotechnol Bioeng ; 116(11): 2803-2814, 2019 11.

Article in En | MEDLINE | ID: mdl-31317525

Subject(s)

Bioreactors; Dependovirus/growth & development; Models, Biological; Animals; Dependovirus/genetics; Sf9 Cells; Spectrometry, Fluorescence; Spodoptera

Key words

adeno-associated virus; chemometrics; fluorescence spectroscopy; insect cell-baculovirus system; process analytical technology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dependovirus / Bioreactors / Models, Biological Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Biotechnol Bioeng Year: 2019 Type: Article Affiliation country: Portugal

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