Your browser doesn't support javascript.
loading
Establishment of a Seronegative Occult Infection With an Active Hepatitis B Virus Reservoir Enriched of Vaccine Escape Mutations in a Vaccinated Infant After Liver Transplantation.
Salpini, Romina; Pietrobattista, Andrea; Piermatteo, Lorenzo; Basso, Maria Sole; Bellocchi, Maria C; Liccardo, Daniela; Carioti, Luca; Francalanci, Paola; Aragri, Marianna; Alkhatib, Mohammed; Scutari, Rossana; Candusso, Manila; Ciotti, Marco; Svicher, Valentina.
Affiliation
  • Salpini R; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Pietrobattista A; Department of Hepatology and Gastroenterology, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Piermatteo L; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Basso MS; Department of Hepatology and Gastroenterology, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Bellocchi MC; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Liccardo D; Department of Hepatology and Gastroenterology, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Carioti L; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Francalanci P; Department of Pathology, Bambino Gesù Children Hospital, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Aragri M; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Alkhatib M; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Scutari R; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
  • Candusso M; Department of Hepatology and Gastroenterology, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Ciotti M; Department of Clinical Microbiology and Virology, Polyclinic Tor Vergata Foundation, Rome, Italy.
  • Svicher V; Department of Experimental Medicine, University of Rome "Tor Vergata,", Rome, Italy.
J Infect Dis ; 220(12): 1935-1939, 2019 11 06.
Article in En | MEDLINE | ID: mdl-31412121
ABSTRACT
We describe the establishment of a seronegative occult hepatitis B virus (HBV) infection (OBI) in a successfully vaccinated infant who underwent liver transplantation from an donor positive for antibody to hepatitis B core antigen (anti-HBc). The use of highly sensitive droplet digital polymerase chain reaction assays revealed a not negligible and transcriptionally active intrahepatic HBV reservoir (circular covalently closed DNA, relaxed circular DNA, and pregenomic RNA 5.6, 2.4, and 1.1 copies/1000 cells, respectively), capable to sustain ongoing viral production and initial liver damage. Next-generation sequencing revealed a peculiar enrichment of hepatitis B surface antigen vaccine-escape mutations that could have played a crucial role in OBI transmission. This clinical case highlights the pathobiological complexity and the diagnostic challenges underlying OBI.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hepatitis B virus / Liver Transplantation / Hepatitis B / Mutation Type of study: Etiology_studies Limits: Child, preschool / Female / Humans Language: En Journal: J Infect Dis Year: 2019 Type: Article Affiliation country: Italy
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hepatitis B virus / Liver Transplantation / Hepatitis B / Mutation Type of study: Etiology_studies Limits: Child, preschool / Female / Humans Language: En Journal: J Infect Dis Year: 2019 Type: Article Affiliation country: Italy