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Rapid Presumptive Identification of Bacillus anthracis Isolates Using the Tetracore RedLine Alert™ Test.
Pillai, Segaran P; Prentice, Kristin W; Ramage, Jason G; DePalma, Lindsay; Sarwar, Jawad; Parameswaran, Nishanth; Bell, Melissa; Plummer, Andrea; Santos, Alan; Singh, Ajay; Pillai, Christine A; Thirunavvukarasu, Nagarajan; Manickam, Gowri; Avila, Julie R; Sharma, Shashi K; Hoffmaster, Alex; Anderson, Kevin; Morse, Stephen A; Venkateswaran, Kodumudi Venkat; Hodge, David R.
Affiliation
  • Pillai SP; Segaran P. Pillai, PhD, is Director, Office of Laboratory Science and Safety, FDA Office of the Commissioner, Department of Health and Human Services, Silver Spring, MD.
  • Prentice KW; Kristin W. Prentice, MS, is an Associate, and Lindsay DePalma, MS, is a Staff Life Scientist; both at Booz Allen Hamilton, Rockville, MD.
  • Ramage JG; Jason G. Ramage, MS, MBA, PMP, is Assistant Vice Chancellor for Research and Innovation and Director of Research Compliance, University of Arkansas, Fayetteville, AR.
  • DePalma L; Kristin W. Prentice, MS, is an Associate, and Lindsay DePalma, MS, is a Staff Life Scientist; both at Booz Allen Hamilton, Rockville, MD.
  • Sarwar J; Jawad Sarwar, MS, is a Senior Research Scientist, and Nishanth Parameswaran is a Research Scientist; both at Omni Array Biotechnology, Rockville, MD.
  • Parameswaran N; Jawad Sarwar, MS, is a Senior Research Scientist, and Nishanth Parameswaran is a Research Scientist; both at Omni Array Biotechnology, Rockville, MD.
  • Bell M; Melissa Bell, MS, is a Microbiologist, and Alex Hoffmaster, PhD, is Chief, Bacterial Special Pathogens Branch; both in the National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA.
  • Plummer A; Andrea Plummer and Alan Santos are Microbiologists, and Kodumudi Venkat Venkateswaran, PhD, is Chief Scientist; all at Tetracore, Inc., Rockville, MD.
  • Santos A; Andrea Plummer and Alan Santos are Microbiologists, and Kodumudi Venkat Venkateswaran, PhD, is Chief Scientist; all at Tetracore, Inc., Rockville, MD.
  • Singh A; Ajay Singh, PhD, is a Research Scientist, Laulima Government Solutions, Contractor Support to USAMRICD Neurobiological Toxicology Branch, Analytical Toxicology Division, Aberdeen Proving Ground, MD.
  • Pillai CA; Christine A. Pillai, Nagarajan Thirunavvukarasu, PhD, and Gowri Manickam, PhD, are ORISE Fellow Research Scientists, and Shashi K. Sharma, PhD, is a Research Microbiologist; all with the FDA Center for Food Safety and Applied Nutrition, Molecular Methods Development Branch, Division of Microbiology,
  • Thirunavvukarasu N; Christine A. Pillai, Nagarajan Thirunavvukarasu, PhD, and Gowri Manickam, PhD, are ORISE Fellow Research Scientists, and Shashi K. Sharma, PhD, is a Research Microbiologist; all with the FDA Center for Food Safety and Applied Nutrition, Molecular Methods Development Branch, Division of Microbiology,
  • Manickam G; Christine A. Pillai, Nagarajan Thirunavvukarasu, PhD, and Gowri Manickam, PhD, are ORISE Fellow Research Scientists, and Shashi K. Sharma, PhD, is a Research Microbiologist; all with the FDA Center for Food Safety and Applied Nutrition, Molecular Methods Development Branch, Division of Microbiology,
  • Avila JR; Julie R. Avila, MS, is a Scientific Associate, Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA.
  • Sharma SK; Christine A. Pillai, Nagarajan Thirunavvukarasu, PhD, and Gowri Manickam, PhD, are ORISE Fellow Research Scientists, and Shashi K. Sharma, PhD, is a Research Microbiologist; all with the FDA Center for Food Safety and Applied Nutrition, Molecular Methods Development Branch, Division of Microbiology,
  • Hoffmaster A; Melissa Bell, MS, is a Microbiologist, and Alex Hoffmaster, PhD, is Chief, Bacterial Special Pathogens Branch; both in the National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA.
  • Anderson K; Kevin Anderson, PhD, and David R. Hodge, PhD, are Program Managers, Science and Technology Directorate, US Department of Homeland Security, Washington, DC.
  • Morse SA; Stephen A. Morse, MSPH, PhD, is a Senior Advisor, CDC Division of Select Agents and Toxins, and is currently with IHRC, Inc., Atlanta, GA.
  • Venkateswaran KV; Andrea Plummer and Alan Santos are Microbiologists, and Kodumudi Venkat Venkateswaran, PhD, is Chief Scientist; all at Tetracore, Inc., Rockville, MD.
  • Hodge DR; Kevin Anderson, PhD, and David R. Hodge, PhD, are Program Managers, Science and Technology Directorate, US Department of Homeland Security, Washington, DC.
Health Secur ; 17(4): 334-343, 2019.
Article in En | MEDLINE | ID: mdl-31433282
ABSTRACT
A comprehensive laboratory evaluation of the Tetracore RedLine Alert test, a lateral flow immunoassay (LFA) for the rapid presumptive identification of Bacillus anthracis, was conducted at 2 different test sites. The study evaluated the sensitivity of this assay using 16 diverse strains of B. anthracis grown on sheep blood agar (SBA) plates. In addition, 83 clinically relevant microorganisms were tested to assess the specificity of the RedLine Alert test. The results indicated that the RedLine Alert test for the presumptive identification of B. anthracis is highly robust, specific, and sensitive. RedLine Alert is a rapid test that has applicability for use in a clinical setting for ruling-in or ruling-out nonhemolytic colonies of Bacillus spp. grown on SBA medium as presumptive isolates of B. anthracis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus anthracis / Immunoassay / Diagnostic Tests, Routine / Anthrax Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Health Secur Year: 2019 Type: Article Affiliation country: Moldova
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus anthracis / Immunoassay / Diagnostic Tests, Routine / Anthrax Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Health Secur Year: 2019 Type: Article Affiliation country: Moldova