Control of DNA replication timing in the 3D genome.
Nat Rev Mol Cell Biol
; 20(12): 721-737, 2019 12.
Article
in En
| MEDLINE
| ID: mdl-31477886
ABSTRACT
The 3D organization of mammalian chromatin was described more than 30 years ago by visualizing sites of DNA synthesis at different times during the S phase of the cell cycle. These early cytogenetic studies revealed structurally stable chromosome domains organized into subnuclear compartments. Active-gene-rich domains in the nuclear interior replicate early, whereas more condensed chromatin domains that are largely at the nuclear and nucleolar periphery replicate later. During the past decade, this spatiotemporal DNA replication programme has been mapped along the genome and found to correlate with epigenetic marks, transcriptional activity and features of 3D genome architecture such as chromosome compartments and topologically associated domains. But the causal relationship between these features and DNA replication timing and the regulatory mechanisms involved have remained an enigma. The recent identification of cis-acting elements regulating the replication time and 3D architecture of individual replication domains and of long non-coding RNAs that coordinate whole chromosome replication provide insights into such mechanisms.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Heterochromatin
/
Genome, Human
/
Cell Cycle
/
Chromatin Assembly and Disassembly
/
DNA Replication Timing
Limits:
Animals
/
Humans
Language:
En
Journal:
Nat Rev Mol Cell Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Type:
Article
Affiliation country:
United States