A small-molecule competitive inhibitor of phosphatidic acid binding by the AAA+ protein NSF/Sec18 blocks the SNARE-priming stage of vacuole fusion.

Sparks, Robert P; Arango, Andres S; Starr, Matthew L; Aboff, Zachary L; Hurst, Logan R; Rivera-Kohr, David A; Zhang, Chi; Harnden, Kevin A; Jenkins, Jermaine L; Guida, Wayne C; Tajkhorshid, Emad; Fratti, Rutilio A

Sparks, Robert P; Arango, Andres S; Starr, Matthew L; Aboff, Zachary L; Hurst, Logan R; Rivera-Kohr, David A; Zhang, Chi; Harnden, Kevin A; Jenkins, Jermaine L; Guida, Wayne C; Tajkhorshid, Emad; Fratti, Rutilio A.

Affiliation

Sparks RP; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Arango AS; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Starr ML; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Aboff ZL; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Hurst LR; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Rivera-Kohr DA; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Zhang C; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Harnden KA; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Jenkins JL; Structural Biology and Biophysics Facility, University of Rochester Medical Center, Rochester, New York 14642.
Guida WC; Department of Chemistry, University of South Florida, Tampa, Florida 336204.
Tajkhorshid E; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Fratti RA; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.

J Biol Chem ; 294(46): 17168-17185, 2019 11 15.

Article in En | MEDLINE | ID: mdl-31515268

Subject(s)

Adenosine Triphosphatases/genetics; Ethylmaleimide/metabolism; Phosphatidic Acids/chemistry; Saccharomyces cerevisiae Proteins/genetics; Small Molecule Libraries/chemistry; Vesicular Transport Proteins/genetics; ATPases Associated with Diverse Cellular Activities/chemistry; ATPases Associated with Diverse Cellular Activities/genetics; Adenosine Triphosphatases/chemistry; Membrane Fusion/drug effects; Membrane Fusion/genetics; Membrane Lipids/chemistry; Membrane Lipids/genetics; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/genetics; Molecular Dynamics Simulation; N-Ethylmaleimide-Sensitive Proteins/chemistry; N-Ethylmaleimide-Sensitive Proteins/genetics; Phosphatidic Acids/antagonists & inhibitors; SNARE Proteins/chemistry; SNARE Proteins/genetics; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/chemistry; Small Molecule Libraries/pharmacology; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/chemistry; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/genetics; Vacuoles/genetics; Vesicular Transport Proteins/chemistry

Key words

NSF; SNARE proteins; Sec17; Sec18; membrane fusion; membrane lipid; membrane trafficking; phosphatidic acid; α-SNAP

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphatidic Acids / Adenosine Triphosphatases / Saccharomyces cerevisiae Proteins / Vesicular Transport Proteins / Ethylmaleimide / Small Molecule Libraries Language: En Journal: J Biol Chem Year: 2019 Type: Article

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