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Mutations in sorghum SBEIIb and SSIIa affect alkali spreading value, starch composition, thermal properties and flour viscosity.
Griebel, Stefanie; Westerman, Richard P; Adeyanju, Adedayo; Addo-Quaye, Charles; Craig, Bruce A; Weil, Clifford F; Cunningham, Suzanne M; Patel, Bhavesh; Campanella, Osvaldo H; Tuinstra, Mitchell R.
Affiliation
  • Griebel S; Department of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
  • Westerman RP; College of Agriculture Administration, Purdue University, West Lafayette, IN, 47907, USA.
  • Adeyanju A; Department of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
  • Addo-Quaye C; Division Natural Sciences and Mathematics, Lewis-Clark State College, Lewiston, ID, 83501, USA.
  • Craig BA; Department of Statistics, Purdue University, West Lafayette, IN, 47907, USA.
  • Weil CF; Department of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
  • Cunningham SM; Department of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
  • Patel B; Whistler Carbohydrate Research Center, Purdue University, West Lafayette, IN, 47907, USA.
  • Campanella OH; Whistler Carbohydrate Research Center, Purdue University, West Lafayette, IN, 47907, USA.
  • Tuinstra MR; Department of Food Science and Technology, The Ohio State University, Columbus, OH, 43210-1007, USA.
Theor Appl Genet ; 132(12): 3357-3374, 2019 Dec.
Article in En | MEDLINE | ID: mdl-31624872
KEY MESSAGE: Seven novel alleles of SBEIIb and one allele of SSIIa co-segregated with the ASV phenotype and contributed to distinct starch quality traits important for food-processing applications. Sorghum is an important food crop for millions of people in Africa and Asia. Whole-genome re-sequencing of sorghum EMS mutants exhibiting an alkali spreading value (ASV) phenotype revealed candidate SNPs in Sobic.004G163700 and Sobic.010G093400. Comparative genomics identified Sobic.010G093400 as a starch synthase IIa and Sobic.004G163700 as a starch branching enzyme IIb. Segregation analyses showed that mutations in Sobic.010G093400 or Sobic.004G163700 co-segregated with the ASV phenotype. Mutants in SSIIa exhibited no change in amylose content but expressed lower final viscosity and lower starch gelatinization temperature (GT) than starches from non-mutant plants. The sbeIIb mutants exhibited significantly higher amylose levels and starch GT and lower viscosity compared to non-mutant starches and ssIIa mutants. Mutations in SBEIIb had a dosage-dependent effect on amylose content. Double mutants of sbeIIb and ssIIa resembled their sbeIIb parent in amylose content, starch thermal properties and viscosity profiles. These variants will provide opportunities to produce sorghum varieties with modified starch end-use qualities important for the beer brewing and baking industries and specialty foods for humans with diabetes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Starch / Sorghum / Flour / Amylose Language: En Journal: Theor Appl Genet Year: 2019 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Starch / Sorghum / Flour / Amylose Language: En Journal: Theor Appl Genet Year: 2019 Type: Article Affiliation country: United States