Dynamic control of plant water use using designed ABA receptor agonists.
Science
; 366(6464)2019 10 25.
Article
in En
| MEDLINE
| ID: mdl-31649167
ABSTRACT
Drought causes crop losses worldwide, and its impact is expected to increase as the world warms. This has motivated the development of small-molecule tools for mitigating the effects of drought on agriculture. We show here that current leads are limited by poor bioactivity in wheat, a widely grown staple crop, and in tomato. To address this limitation, we combined virtual screening, x-ray crystallography, and structure-guided design to develop opabactin (OP), an abscisic acid (ABA) mimic with up to an approximately sevenfold increase in receptor affinity relative to ABA and up to 10-fold greater activity in vivo. Studies in Arabidopsis thaliana reveal a role of the type III receptor PYRABACTIN RESISTANCE-LIKE 2 for the antitranspirant efficacy of OP. Thus, virtual screening and structure-guided optimization yielded newly discovered agonists for manipulating crop abiotic stress tolerance and water use.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stress, Physiological
/
Benzamides
/
Water
/
Arabidopsis
/
Abscisic Acid
/
Receptors, Cell Surface
/
Cyclohexanes
/
Arabidopsis Proteins
/
Hormones
Language:
En
Journal:
Science
Year:
2019
Type:
Article
Affiliation country:
United States