Thermal stress causes nuclear and cellular abnormalities of peripheral erythrocytes in Indian major carp, rohu Labeo rohita.
J Therm Biol
; 86: 102450, 2019 Dec.
Article
in En
| MEDLINE
| ID: mdl-31789238
ABSTRACT
Rise of water temperature as a consequence of global warming is anticipated to affect the physiological activities of fish, especially in tropical regions. In the present experiment, we exposed the Indian major carp, rohu Labeo rohita to three different temperature regimes (30⯰C as control and 33⯰C and 36⯰C) for 60 days and observed the effects of these temperature on major hemato-biochemical indices (Hemoglobin; Hb, Red blood cell; RBC, White blood cell; WBC and blood glucose levels), erythrocytic nuclear abnormalities (ENA), and erythrocytic cellular abnormalities (ECA) of peripheral erythrocytes along with the formation of differential leucocytes in the blood. Fish were sacrificed at day 7, 15, 30 and 60 after the start of exposure to the temperature regimes. Hb decreased significantly on days 7 and 15â¯at 36⯰C. Throughout the study period, the decrease of RBC and increase of WBC were significant at 36⯰C. Blood glucose level increased significantly initially at day 7 but decreased significantly at day 60â¯at 36⯰C. Frequencies of ENA (binucleated, nuclear bud, nuclear bridge, karyopyknosis and notched nuclei) and ECA (twin, fusion, echinocytic, spindle, tear drop and elongated shaped) were significantly increased at the highest temperature (36⯰C) at almost all of the sampling days. In the case of differential leucocyte count, high temperature caused a significant increase in the number of neutrophils and a significant decrease in the number of lymphocytes. Overall, these results indicate that chronic exposure to high temperature (36⯰C) induces a number of stress responses in rohu and that temperature should be kept below 36⯰C in the aquaculture setting to avoid damage to the fish.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carps
/
Heat Stress Disorders
/
Fish Diseases
/
Hot Temperature
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
J Therm Biol
Year:
2019
Type:
Article
Affiliation country:
Bangladesh