Antifungal activity of different molecular weight chitosans against planktonic cells and biofilm of Sporothrix brasiliensis.
Int J Biol Macromol
; 143: 341-348, 2020 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-31812752
ABSTRACT
Sporotrichosis, caused by Sporothrix schenckii complex species, is the most prevalent subcutaneous mycosis in many areas of Latin America. Chitosan has been used as an antifungal agent; however the effects of the molecular weight (MW) of chitosan (i.e. high (HMW), medium (MMW) and low (LMW) molecular weight chitosan) on S. brasiliensis has not been well described, particularly on biofilms. Effects on the planktonic form activity of S. brasiliensis were quantified by broth microdilution, while anti-biofilm activity was quantified by measuring metabolic activity via XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide and biomass formation (crystal violet). The molecular weight of chitosan modulated its effect on the planktonic form of S. brasiliensis, presenting lower MIC values for LMW chitosan. With regards both the adhesive and mature phases of biofilm, the LMW chitosan reduced biomass and metabolic activity most effectively. This study confirms the effects of the molecular weight and deacetylation degree of chitosan on its antifungal properties for potentially pathogenic fungi.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sporothrix
/
Biofilms
/
Chitosan
Limits:
Humans
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Int J Biol Macromol
Year:
2020
Type:
Article
Affiliation country:
Brazil