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Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.
Villette, Vincent; Chavarha, Mariya; Dimov, Ivan K; Bradley, Jonathan; Pradhan, Lagnajeet; Mathieu, Benjamin; Evans, Stephen W; Chamberland, Simon; Shi, Dongqing; Yang, Renzhi; Kim, Benjamin B; Ayon, Annick; Jalil, Abdelali; St-Pierre, François; Schnitzer, Mark J; Bi, Guoqiang; Toth, Katalin; Ding, Jun; Dieudonné, Stéphane; Lin, Michael Z.
Affiliation
  • Villette V; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Chavarha M; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Dimov IK; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA; CNC Program, Stanford University, Stanford, CA 94305, USA.
  • Bradley J; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Pradhan L; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA; CNC Program, Stanford University, Stanford, CA 94305, USA.
  • Mathieu B; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Evans SW; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Chamberland S; Department of Psychiatry and Neuroscience, CERVO Brain Research Centre, Université Laval, Quebec City, QC G1J 2G3, Canada.
  • Shi D; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA; School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
  • Yang R; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA; Biology PhD Program, Stanford University, Stanford, CA 94305, USA.
  • Kim BB; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Ayon A; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Jalil A; Université de Paris, SPPIN - Saints-Pères Paris Institute for the Neurosciences, CNRS, Paris F-75006, France.
  • St-Pierre F; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
  • Schnitzer MJ; CNC Program, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Bi G; School of Life Sciences, University of Science and Technology of China, Hefei 230026, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 20031, China.
  • Toth K; Department of Psychiatry and Neuroscience, CERVO Brain Research Centre, Université Laval, Quebec City, QC G1J 2G3, Canada.
  • Ding J; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
  • Dieudonné S; Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France. Electronic address: dieudon@biologie.ens.fr.
  • Lin MZ; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. Electronic address: mzlin@stanford.edu.
Cell ; 179(7): 1590-1608.e23, 2019 12 12.
Article in En | MEDLINE | ID: mdl-31835034
Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased stability and sensitivity. Combining a soma-targeted ASAP3 variant and ULoVE, we show single-trial tracking of spikes and subthreshold events for minutes in deep locations, with subcellular resolution and with repeated sampling over days. In the visual cortex, we use soma-targeted ASAP3 to illustrate cell-type-dependent subthreshold modulation by locomotion. Thus, ASAP3 and ULoVE enable high-speed optical recording of electrical activity in genetically defined neurons at deep locations during awake behavior.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Theta Rhythm / Wakefulness / Brain / GTPase-Activating Proteins / Microscopy, Fluorescence, Multiphoton / Optogenetics Limits: Animals / Female / Humans / Male Language: En Journal: Cell Year: 2019 Type: Article Affiliation country: France
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Theta Rhythm / Wakefulness / Brain / GTPase-Activating Proteins / Microscopy, Fluorescence, Multiphoton / Optogenetics Limits: Animals / Female / Humans / Male Language: En Journal: Cell Year: 2019 Type: Article Affiliation country: France