Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC.
Methods Enzymol
; 632: 193-213, 2020.
Article
in En
| MEDLINE
| ID: mdl-32000896
ABSTRACT
Immunotherapy has emerged as a potent alternative for cancer treatment, unfortunately, the clinical benefit remains limited to few patients and immunotherapy resistance due to immunosuppressive tumor microenvironment represents the major reason of such a failure. Arginase-1 is one of the enzymes contributing to the establishment of such immunosuppression. Among the human immune cells, polymorphonuclear cells (PMNs) represent the major source of arginase-1, while myeloid-derived suppressor cells (MDSCs) are the main arginase-1 producing cells in mice. Due to arginase-1 potential impact in dampening the immune response, there is a growing interest in assaying arginase-1 levels and functions. Thus, in this chapter we propose how to evaluate the expression and activity of arginase in human peripheral blood-derived PMNs and in MDSCs isolated from tumor-bearing mice.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginase
/
Myeloid-Derived Suppressor Cells
/
Neutrophils
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Enzymol
Year:
2020
Type:
Article
Affiliation country:
Italy