Identification of stable genes in the corpus luteum of lactating Holstein cows in pregnancy and luteolysis: Implications for selection of reverse-transcription quantitative PCR reference genes.

Mezera, M A; Li, W; Edwards, A J; Koch, D J; Beard, A D; Wiltbank, M C

Mezera, M A; Li, W; Edwards, A J; Koch, D J; Beard, A D; Wiltbank, M C.

Affiliation

Mezera MA; Department of Dairy Science, University of Wisconsin, Madison 53706; Endocrinology and Reproductive Physiology Program, University of Wisconsin, Madison 53706.
Li W; USDA Dairy Forage Research Center, Madison, WI 53706. Electronic address: wenli.li@usda.gov.
Edwards AJ; USDA Dairy Forage Research Center, Madison, WI 53706.
Koch DJ; USDA Dairy Forage Research Center, Madison, WI 53706.
Beard AD; Department of Dairy Science, University of Wisconsin, Madison 53706; Endocrinology and Reproductive Physiology Program, University of Wisconsin, Madison 53706.
Wiltbank MC; Department of Dairy Science, University of Wisconsin, Madison 53706.

J Dairy Sci ; 103(5): 4846-4857, 2020 May.

Article in En | MEDLINE | ID: mdl-32229123

Subject(s)

Cattle/genetics; Corpus Luteum/metabolism; Gene Expression; Lactation; Luteolysis/metabolism; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Animals; Base Sequence; Cattle/metabolism; Corpus Luteum Maintenance; Female; Pregnancy; RNA, Messenger/metabolism; Sequence Analysis, RNA

Key words

corpus luteum; dairy cow; reference gene; reverse-transcription quantitative PCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lactation / Cattle / Gene Expression / Corpus Luteum / Luteolysis / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Pregnancy Language: En Journal: J Dairy Sci Year: 2020 Type: Article

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