The role of ligand-receptor interactions in visual detection of HepG2 cells using a liquid crystal microdroplet-based biosensor.
J Mater Chem B
; 3(44): 8659-8669, 2015 Nov 28.
Article
in En
| MEDLINE
| ID: mdl-32262723
ABSTRACT
Liquid crystal (LC) microdroplets have been prepared for visual detection of HepG2 cells using 4-cyano-4'-pentyl biphenyl molecules in the presence of sodium dodecyl sulfate as a mediator and ß-galactose-conjugated poly(styrene-b-acrylic acid) block copolymer (PS-b-PA-G) as a modifier of LC-water interfaces. To clarify the effect of ß-galactose-containing ligands on the orientational transitions of LC microdroplets, maltotriose as a ligand simulant was conjugated to poly(styrene-b-acrylic acid) and used as a LC modifier. The interaction of HepG2 cells with the ß-galactose-conjugated block copolymer was effective in causing orientational transitions, from radial to bipolar, in LC microdroplets, whereas interactions of HepG2 cells with maltotriose-conjugated block copolymers were ineffective in inducing orientational transitions in LC microdroplets. To confirm the necessity of the PS segment of the block copolymer for transmitting the ligand-receptor interaction forces from the interface to the core of the LC microdroplets, ß-galactose-conjugated block copolymers (PS-b-PA-G) and homopolymers (PVLA) were synthesized and used to prepare LC microdroplets. The LC microdroplets containing a ß-galactose-conjugated homopolymer did not show orientational transitions upon contact with HepG2 cells. However, LC microdroplets containing a ß-galactose-conjugated block copolymer showed orientational transitions from radial to bipolar, indicating that the polystyrene segment in the amphiphilic block copolymer is essential for the effective transmission of ligand-receptor interactions to the core of LC microdroplets. ß-Galactose anchored LC microdroplets were able to detect 1.0 ± 0.1 HepG2 cells per µm2 of the test cell and had shown significantly high reproducibility (p < 0.05, n = 3). The configurational transition in LC microdroplets that was dependent on ligand-receptor interactions was used to develop a LC microdroplet-based biosensor for the detection of HepG2 cells in biological fluids.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Mater Chem B
Year:
2015
Type:
Article