Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments.
Breed Sci
; 70(1): 128-134, 2020 Mar.
Article
in En
| MEDLINE
| ID: mdl-32351312
ABSTRACT
Buckwheat (Fagopyrum esculentum) is cultivated worldwide and its flour is used in a variety of food products. Although functional analyses of genes in buckwheat are highly desired, reliable methods to do it have yet to be developed. In this study we established a simple and efficient transient gene expression system using buckwheat protoplasts isolated from young hypocotyls using 96-well plates as a high-throughput platform. The transformation efficiency was comparable with that of similar systems, such as Arabidopsis mesophyll protoplasts. Stable results were obtained in a typical example of the experiment to examine transcription factor activity. This system shows potential for the large-scale analysis of gene function using protoplast isolated from fewer and younger plants than the conventional system and may provide novel information for efficient buckwheat breeding.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Breed Sci
Year:
2020
Type:
Article
Affiliation country:
Japan