Curcumin potentiates laryngeal squamous carcinoma radiosensitivity via NF-ΚB inhibition by suppressing IKKγ expression.
J Recept Signal Transduct Res
; 40(6): 541-549, 2020 Dec.
Article
in En
| MEDLINE
| ID: mdl-32515250
ABSTRACT
Context Curcumin has shown efficacy in promoting radiosensitivity combined with radiotherapy. However, the role and mechanism of curcumin on radiosensitivity in laryngeal squamous cell cancer (LSCC) is largely unknown.Objective:
The aim of our study is to explore the role of IKKγ-NF-κB signaling in curcumin enhancing LSCC cell radiosensitivity in vitro.Materials andmethods:
Curcumin and X-ray were used to induce cell DNA damage and apoptosis, or inhibit cell clone formation. IKKγ siRNA and plasmid were used to change IKKγ expression. The CCK8 assay was used to detect cell viability. Clone formation ability was analyzed using a clonogenic assay, cell apoptosis was examined using flow cytometry, an immunofluorescence assay was used to detect DNA damage, while mRNA and protein levels were assayed using real time PCR and western blotting, respectively.Results:
Curcumin significantly enhanced irradiation-induced DNA damage and apoptosis, while weakening clone-forming abilities of LSCC cell line Hep2 and Hep2-max. Compared to Hep2 cells, Hep2-max cells are more sensitive to curcumin post-irradiation. Curcumin suppressed irradiation-induced NF-κB activation by suppressing IKKγ expression, but not IKKα and IKKß. Overexpression of IKKγ decreased irradiation-induced DNA damage and apoptosis, while promoting clone-forming abilities of Hep2 and Hep2-max cells. IKKγ overexpression further increased expression of NF-κB downstream genes, Bcl-XL, Bcl-2, and cyclin D1. Conversely, IKKγ silencing enhanced irradiation-induced DNA damage and apoptosis, but promoted clone formation in Hep2 and Hep2-max cells. Additionally, IKKγ silencing inhibited expression of Bcl-XL, Bcl-2, and cyclin D1.Conclusions:
Curcumin enhances LSCC radiosensitivity via NF-ΚB inhibition by suppressing IKKγ expression.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation-Sensitizing Agents
/
Radiation Tolerance
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Carcinoma, Squamous Cell
/
Laryngeal Neoplasms
/
NF-kappa B
/
Curcumin
/
I-kappa B Kinase
Limits:
Humans
Language:
En
Journal:
J Recept Signal Transduct Res
Journal subject:
BIOQUIMICA
/
FISIOLOGIA
Year:
2020
Type:
Article