Bulk and single-cell gene expression analyses reveal aging human choriocapillaris has pro-inflammatory phenotype.
Microvasc Res
; 131: 104031, 2020 09.
Article
in En
| MEDLINE
| ID: mdl-32531351
ABSTRACT
The human choroidal vasculature is subject to age-related structural and gene expression changes implicated in age-related macular degeneration (AMD). In this study, we performed both bulk and single-cell RNA sequencing on infant (n = 4 for bulk experiments, n = 2 for single-cell experiments) and adult (n = 13 for bulk experiments, n = 6 for single-cell experiments) human donors to characterize how choroidal gene expression changes with age. Differential expression analysis revealed that aged choroidal samples were enriched in genes encoding pro-inflammatory transcription factors and leukocyte transendothelial cell migration adhesion proteins. Such genes were observed to be differentially expressed specifically within choroidal endothelial cells at the single-cell level. Immunohistochemistry experiments support transcriptional findings that CD34 is elevated in infant choriocapillaris endothelial cells while ICAM-1 is enriched in adults. These results suggest several potential drivers of the pro-inflammatory vascular phenotype observed with advancing age.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aging
/
Choroid
/
Sequence Analysis, RNA
/
Inflammation Mediators
/
Endothelial Cells
/
Single-Cell Analysis
/
Inflammation
/
Macular Degeneration
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Infant
/
Male
/
Middle aged
/
Newborn
Language:
En
Journal:
Microvasc Res
Year:
2020
Type:
Article
Affiliation country:
United States