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Expression and characterization of a novel organic solvent tolerant protease from Bacillus sphaericus DS11.
Wu, Xincai; Ahmed, Sibtain; Cui, Xiaolin; Hang, Jiahao; Wang, Shujun; Liu, Shu; Fang, Yaowei.
Affiliation
  • Wu X; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China.
  • Ahmed S; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China.
  • Cui X; Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
  • Hang J; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China.
  • Wang S; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China.
  • Liu S; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China.
  • Fang Y; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China.
Prep Biochem Biotechnol ; 51(1): 28-34, 2021.
Article in En | MEDLINE | ID: mdl-32633612
ABSTRACT
Organic solvent-tolerant proteases have many applications in the synthesis of peptides. In this study, we have developed a low-cost and convenient method to produce highly concentrated organic solvent-tolerant protease. Organic solvent tolerant protease (OSP) gene from Bacillus sphaericus DS11 was cloned and expressed in Bacillus subtilis WB800. The optimum pH of the recombinant protease was 9.0. The optimum temperature of the recombinant protease was 40 °C. The recombinant protease was purified by ethanol with the yield of (87.33%). The yield of OSP enriched by ethanol was higher than that of by Ni-chelating affinity chromatography, which indicated that precipitation of the recombinant OSP with ethanol is a relatively low-cost and fast method for organic solvent -tolerant protease preparation. These results showed that this enzyme could be very useful in different industrial applications.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Hydrolases / Solvents / Bacillaceae / Bacillus subtilis / Bacterial Proteins Language: En Journal: Prep Biochem Biotechnol Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2021 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Hydrolases / Solvents / Bacillaceae / Bacillus subtilis / Bacterial Proteins Language: En Journal: Prep Biochem Biotechnol Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2021 Type: Article Affiliation country: China