Chondrogenic differentiation followed IGFBP3 loss in human endometrial mesenchymal stem cells.
Biochem Biophys Res Commun
; 531(2): 133-139, 2020 10 15.
Article
in En
| MEDLINE
| ID: mdl-32782147
ABSTRACT
Insulin-like growth factor binding protein 3 (IGFBP3) is a multifunctional protein, able either to stimulate the cell growth or to promote apoptosis. In particular, IGFBP3 plays significant role in propagation of stress-induced senescence in human endometrium-derived mesenchymal stem cells (MESCs) (Vassilieva et al., 2020). We undertook CRISPR/Cas9-mediated IGFBP3 knockout in an effort to decelerate stress-induced senescence in MESCs, but, unexpectedly, IGFBP3-knockout MESCs culture acquired chondrocyte-like features, such as cell condensation and aggregation. We revealed that IGFBP3-knockout MESCs completely lost CD73 and CD90 MESCs positive surface markers, and significantly decreased expression of CD105 and CD146 MESCs positive surface markers. In addition, we found IGFBP3-knockout MESCs aggregates positively stained for Alcian Blue. We also detected expression of collagen type II in IGFBP3-knockout MESCs. The obtained results indicate that MESCs lost stemness after IGFBP3-knockout and underwent differentiation toward chondrogenic lineage. Our findings can enlighten IGFBP3 role in regulation of MESCs chondrogenesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
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Insulin-Like Growth Factor Binding Protein 3
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Chondrogenesis
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Endometrium
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Mesenchymal Stem Cells
Limits:
Female
/
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2020
Type:
Article