Improving Transgenesis Efficiency and CRISPR-Associated Tools Through Codon Optimization and Native Intron Addition in <i>Pristionchus</i> Nematodes.

Han, Ziduan; Lo, Wen-Sui; Lightfoot, James W; Witte, Hanh; Sun, Shuai; Sommer, Ralf J

Improving Transgenesis Efficiency and CRISPR-Associated Tools Through Codon Optimization and Native Intron Addition in Pristionchus Nematodes.

Han, Ziduan; Lo, Wen-Sui; Lightfoot, James W; Witte, Hanh; Sun, Shuai; Sommer, Ralf J.

Affiliation

Han Z; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Lo WS; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Lightfoot JW; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Witte H; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Sun S; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Sommer RJ; Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany ralf.sommer@tuebingen.mpg.de.

Genetics ; 216(4): 947-956, 2020 12.

Article in En | MEDLINE | ID: mdl-33060138

Subject(s)

CRISPR-Cas Systems; Codon Usage; Gene Editing/methods; Rhabditida/genetics; Transgenes; Animals; Gene Editing/standards; Introns

Key words

C. elegans; CRISPR editing; P. pacificus; codon usage bias; intron-mediated enhancement; nematodes; parasitic nematodes; transgenesis

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rhabditida / Transgenes / CRISPR-Cas Systems / Gene Editing / Codon Usage Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Genetics Year: 2020 Type: Article Affiliation country: Germany

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