[Biological Characteristics of Immunophenotype, Stem Cell Gene and Colony Forming Ability in Side-Population Cells of Jeko-1 Cell Line].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 28(5): 1558-1562, 2020 Oct.
Article
in Zh
| MEDLINE
| ID: mdl-33067954
ABSTRACT
AbstractObjective:
To investigate the in vitro biological characteristics of side-population(SP) cells in mantle cell lymphoma(MCL).METHODS:
The SP cells in JeKo-1 cells were enriched by continuous culture and sorted by flow cytometryï¼FCMï¼, and the expression of CD19 and IgM were analyzed. The differences of gene expression and their self-renewal ability between SP cells and non-SP cells were estimated by qRT-PCR and colony-forming assay(CFA) respectively.RESULTS:
SP cells in JeKo-1 cells could be enriched by continuous sorting and in vitro cultureing, and it was found that CD19-/IgM- accounted for (2.79±0.82)%,CD19-/IgM+accounted for (70.99±13.61)%,CD19+/IgM-accounted for (0.55±0.25)%, and CD19+/IgM+accounted for (25.67±14)%. Bmi-1,CD133, C-MYC and Nanog genes were highly expressed in SP cells, but were low expressed in non-SP cells (P<0.05). Compared with non-SP cells, SP cells had stronger colony forming ability (P<0.05).CONCLUSION:
JeKo-1 cell line of mantle cell lymphoma contains SP cells, which can be enriched by continuous sorting and culture for research. The SP cells in JeKo-1 contain four different cell populations with different immunophenotypesï¼ CD19-/IgM-ãCD19-/IgM+ãCD19+/IgM-ãCD19+/IgM+, and show stronger gene expression of stem cells and self-renewal ability in vitro.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neoplastic Stem Cells
/
Side-Population Cells
Language:
Zh
Journal:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Journal subject:
HEMATOLOGIA
Year:
2020
Type:
Article
Affiliation country:
China