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Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia.
Bras, Anne E; Osmani, Zgjim; de Haas, Valerie; Jongen-Lavrencic, Mojca; Te Marvelde, Jeroen G; Zwaan, C Michel; Mejstrikova, Ester; Fernandez, Paula; Szczepanski, Tomasz; Orfao, Alberto; van Dongen, Jacques J M; van der Velden, Vincent H J.
Affiliation
  • Bras AE; Laboratory Medical Immunology (LMI), Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • Osmani Z; Laboratory Medical Immunology (LMI), Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • de Haas V; Dutch Childhood Oncology Group, Utrecht, the Netherlands.
  • Jongen-Lavrencic M; Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands.
  • Te Marvelde JG; Department of Hematology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • Zwaan CM; Laboratory Medical Immunology (LMI), Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • Mejstrikova E; Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands.
  • Fernandez P; Department of Pediatric Oncology/Hematology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
  • Szczepanski T; CLIP-Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, University Hospital Motol, Prague, Czech Republic.
  • Orfao A; Institute for Laboratory Medicine, Kantonsspital Aarau AG, Aarau, Switzerland.
  • van Dongen JJM; Department of Pediatric Hematology and Oncology, Zabrze, Medical University of Silesia (SUM), Katowice, Poland.
  • van der Velden VHJ; Cancer Research Center (IBMCC-CSIC), Department of Medicine and Cytometry Service, University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
Br J Haematol ; 193(5): 922-927, 2021 06.
Article in En | MEDLINE | ID: mdl-33161592
Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukemia / Immunophenotyping / Peroxidase / Flow Cytometry / Neoplasm Proteins Type of study: Clinical_trials / Diagnostic_studies / Guideline Limits: Female / Humans / Male Language: En Journal: Br J Haematol Year: 2021 Type: Article Affiliation country: Netherlands
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukemia / Immunophenotyping / Peroxidase / Flow Cytometry / Neoplasm Proteins Type of study: Clinical_trials / Diagnostic_studies / Guideline Limits: Female / Humans / Male Language: En Journal: Br J Haematol Year: 2021 Type: Article Affiliation country: Netherlands