A Shift Towards an Immature Myeloid Profile in Peripheral Blood of Critically Ill COVID-19 Patients.
Arch Med Res
; 52(3): 311-323, 2021 04.
Article
in En
| MEDLINE
| ID: mdl-33248817
ABSTRACT
BACKGROUND:
SARS-CoV-2, the etiological agent causing COVID-19, has infected more than 27 million people with over 894000 deaths worldwide since its emergence in December 2019. Factors for severe diseases, such as diabetes, hypertension, and obesity have been identified however, the precise pathogenesis is poorly understood. To understand its pathophysiology and to develop effective therapeutic strategies, it is essential to define the prevailing immune cellular subsets.METHODS:
We performed whole circulating immune cells scRNAseq from five critically ill COVID-19 patients, trajectory and gene ontology analysis.RESULTS:
Immature myeloid populations, such as promyelocytes-myelocytes, metamyelocytes, band neutrophils, monocytoid precursors, and activated monocytes predominated. The trajectory with pseudotime analysis supported the finding of immature cell states. While the gene ontology showed myeloid cell activation in immune response, DNA and RNA processing, defense response to the virus, and response to type 1 interferon. Lymphoid lineage was scarce. Expression of genes such as C/EBPß, IRF1and FOSL2 potentially suggests the induction of trained immunity.CONCLUSIONS:
Our results uncover transcriptomic profiles related to immature myeloid lineages and suggest the potential induction of trained immunity.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myeloid Cells
/
COVID-19
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Arch Med Res
Journal subject:
MEDICINA
Year:
2021
Type:
Article