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Comprehensive analysis of TCR repertoire in COVID-19 using single cell sequencing.
Wang, Pingping; Jin, Xiyun; Zhou, Wenyang; Luo, Meng; Xu, Zhaochun; Xu, Chang; Li, Yiqun; Ma, Kexin; Cao, Huimin; Huang, Yan; Xue, Guangfu; Jin, Shuilin; Nie, Huan; Jiang, Qinghua.
Affiliation
  • Wang P; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Jin X; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Zhou W; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Luo M; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Xu Z; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Xu C; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Li Y; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Ma K; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Cao H; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Huang Y; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Xue G; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China.
  • Jin S; School of Mathematics, Harbin Institute of Technology, Harbin 150000, China.
  • Nie H; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China. Electronic address: nh1212@hit.edu.cn.
  • Jiang Q; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China; Key Laboratory of Biological Big Data (Harbin Institute of Technology), Ministry of Education, China. Electronic address: qhjiang@hit.edu.cn.
Genomics ; 113(2): 456-462, 2021 03.
Article in En | MEDLINE | ID: mdl-33383142
T-cell receptor (TCR) is crucial in T cell-mediated virus clearance. To date, TCR bias has been observed in various diseases. However, studies on the TCR repertoire of COVID-19 patients are lacking. Here, we used single-cell V(D)J sequencing to conduct comparative analyses of TCR repertoire between 12 COVID-19 patients and 6 healthy controls, as well as other virus-infected samples. We observed distinct T cell clonal expansion in COVID-19. Further analysis of VJ gene combination revealed 6 VJ pairs significantly increased, while 139 pairs significantly decreased in COVID-19 patients. When considering the VJ combination of α and ß chains at the same time, the combination with the highest frequency on COVID-19 was TRAV12-2-J27-TRBV7-9-J2-3. Besides, preferential usage of V and J gene segments was also observed in samples infected by different viruses. Our study provides novel insights on TCR in COVID-19, which contribute to our understanding of the immune response induced by SARS-CoV-2.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Antigen, T-Cell / Single-Cell Analysis / High-Throughput Nucleotide Sequencing / SARS-CoV-2 / COVID-19 Limits: Female / Humans / Male Language: En Journal: Genomics Journal subject: GENETICA Year: 2021 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Antigen, T-Cell / Single-Cell Analysis / High-Throughput Nucleotide Sequencing / SARS-CoV-2 / COVID-19 Limits: Female / Humans / Male Language: En Journal: Genomics Journal subject: GENETICA Year: 2021 Type: Article Affiliation country: China