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Oral immunization with recombinant Lactobacillus plantarum expressing Nudix hydrolase and 43 kDa proteins confers protection against Trichinella spiralis in BALB/c mice.
Wang, Dan; Liu, Qiong; Jiang, Yan-Long; Huang, Hai-Bin; Li, Jun-Yi; Pan, Tian-Xu; Wang, Nan; Yang, Wen-Tao; Cao, Xin; Zeng, Yan; Shi, Chun-Wei; Wang, Jian-Zhong; Yang, Gui-Lian; Zhao, Quan; Wang, Chun-Feng.
Affiliation
  • Wang D; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Liu Q; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Jiang YL; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Huang HB; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Li JY; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Pan TX; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Wang N; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Yang WT; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Cao X; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Zeng Y; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Shi CW; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Wang JZ; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Yang GL; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
  • Zhao Q; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China. Electronic address: zhaoquan0825@163.com.
  • Wang CF; College of Veterinary Medicine, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, China; Key Laborator
Acta Trop ; 220: 105947, 2021 Aug.
Article in En | MEDLINE | ID: mdl-33971160
Trichinellosis is a significant food-borne zoonotic parasitic disease caused by parasite Trichinella. Given the side effects of anti-Trichinella drugs (e.g., Mebendazole) aroused in the course of treatments, an effective vaccine against the parasite is called for. The therapies available to date are in most instances targeting a single stage of Trichinella, resulting in an incomplete protective immunity against the parasite in terms of the complexity of its developmental stages. In this study, a recombinant dual-expression double anchor vector NC8-pLp-TsNd-S-pgsA'-gp43 was constructed carrying two antigen genes from Trichinella spiralis (T. spiralis), encoding the gp43 and T. spiralis Nudix hydrolase (TsNd) proteins which were mainly expressed in muscle larva (ML) and intestinal infective larva stages of the parasite respectively. These two proteins were to be expressed by Lactobacillus plantarum NC8 (L. plantarum NC8) which was designed to express the two anchored peptides, a truncated poly-γ-glutamic acid synthetase A (pgsA') and the surface layer protein of Lactobacillus acidophilus (SlpA), on its surface for attaching expressed foreign proteins. Oral immunization with the above recombinant vaccine induced higher levels of specific serum IgG and mucosal secretory IgA (SIgA) in BALB/c mice. In addition, cytokines, interferon-γ (IFN- γ), interleukin-4 (IL-4) and IL-17 released by lymphocytes, and CD4+ levels displayed on the surfaces of splenic and mesenteric lymph cells were significantly enhanced by the vaccination. Moreover, after larval challenges, a 75.67 % reduction of adult worms (AW) at 7 days post-infection (dpi) and 57.14 % reduction of ML at 42 dpi were observed in mice immunized with the recombinant vaccine. Furthermore, this oral vaccination reduced the counts of encysted larvae presented in tongue and masseter muscles after infected with T. spiralis in mice. The overall results demonstrated that the recombinant vaccine developed in this study could induce specific humoral, mucosal, and cellular immune responses, and provides protections against different stages (adult worms and muscle larva) of T. spiralis infections in BALB/c mice, which could make it a promising oral vaccine candidate against trichinellosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrophosphatases / Trichinellosis / Vaccines, Synthetic / Vaccination / Trichinella spiralis / Lactobacillus plantarum Limits: Animals Language: En Journal: Acta Trop Year: 2021 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrophosphatases / Trichinellosis / Vaccines, Synthetic / Vaccination / Trichinella spiralis / Lactobacillus plantarum Limits: Animals Language: En Journal: Acta Trop Year: 2021 Type: Article