The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling.
Cell
; 184(13): 3528-3541.e12, 2021 06 24.
Article
in En
| MEDLINE
| ID: mdl-33984278
ABSTRACT
Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane. The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11-dependent Ca2+ influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resistosome acts as a calcium-permeable cation channel to trigger immunity and cell death.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Signal Transduction
/
Carrier Proteins
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Calcium
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Arabidopsis
/
Arabidopsis Proteins
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Plant Immunity
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Disease Resistance
Limits:
Animals
Language:
En
Journal:
Cell
Year:
2021
Type:
Article
Affiliation country:
China