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Nanofluidics for Simultaneous Size and Charge Profiling of Extracellular Vesicles.
Hosseini, Imman I; Liu, Zezhou; Capaldi, Xavier; AbdelFatah, Tamer; Montermini, Laura; Rak, Janusz; Reisner, Walter; Mahshid, Sara.
Affiliation
  • Hosseini II; Department of Bioengineering, McGill University, 3775 Rue University, Montreal, Quebec H3A 2B4, Canada.
  • Liu Z; Department of Physics, McGill University, 3600 Rue University, Montreal, Quebec H3A 2T8, Canada.
  • Capaldi X; Department of Physics, McGill University, 3600 Rue University, Montreal, Quebec H3A 2T8, Canada.
  • AbdelFatah T; Department of Bioengineering, McGill University, 3775 Rue University, Montreal, Quebec H3A 2B4, Canada.
  • Montermini L; Research Institute of the McGill University Health Centre, 1001 Decarie Boul., Montreal, Quebec H4A 3J1, Canada.
  • Rak J; Department of Pediatrics, McGill University, Research Institute of the McGill University Health Centre, 1001 Decarie Boul., Montreal, Quebec H4A 3J1, Canada.
  • Reisner W; Department of Physics, McGill University, 3600 Rue University, Montreal, Quebec H3A 2T8, Canada.
  • Mahshid S; Department of Bioengineering, McGill University, 3775 Rue University, Montreal, Quebec H3A 2B4, Canada.
Nano Lett ; 21(12): 4895-4902, 2021 06 23.
Article in En | MEDLINE | ID: mdl-34061534
ABSTRACT
Extracellular vesicles (EVs) are cell-derived membrane structures that circulate in body fluids and show considerable potential for noninvasive diagnosis. EVs possess surface chemistries and encapsulated molecular cargo that reflect the physiological state of cells from which they originate, including the presence of disease. In order to fully harness the diagnostic potential of EVs, there is a critical need for technologies that can profile large EV populations without sacrificing single EV level detail by averaging over multiple EVs. Here we use a nanofluidic device with tunable confinement to trap EVs in a free-energy landscape that modulates vesicle dynamics in a manner dependent on EV size and charge. As proof-of-principle, we perform size and charge profiling of a population of EVs extracted from human glioblastoma astrocytoma (U373) and normal human astrocytoma (NHA) cell lines.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glioblastoma / Extracellular Vesicles Limits: Humans Language: En Journal: Nano Lett Year: 2021 Type: Article Affiliation country: Canada
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glioblastoma / Extracellular Vesicles Limits: Humans Language: En Journal: Nano Lett Year: 2021 Type: Article Affiliation country: Canada