Generation of human induced pluripotent stem cell lines from 2 patients with MIRAGE syndrome.
Stem Cell Res
; 54: 102417, 2021 07.
Article
in En
| MEDLINE
| ID: mdl-34119956
ABSTRACT
MIRAGE syndrome is a multisystem disorder caused by mutations in SAMD9 (sterile α motif domain-containing protein 9) with a high mortality in the first decade of life. We generated 2 human induced pluripotent stem cell lines from male children diagnosed with MIRAGE syndrome. The cell lines were generated from fibroblasts by integration-free reprogramming using the Sendai virus. Both cell lines were fully characterized regarding their pluripotent identity and differentiation potential, and quality controlled for karyotypic integrity, cell line identity and clearance of reprogramming vectors. The generated cell lines represent a valuable tool to study the disease mechanism of MIRAGE syndrome.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Induced Pluripotent Stem Cells
Limits:
Child
/
Humans
/
Male
Language:
En
Journal:
Stem Cell Res
Year:
2021
Type:
Article
Affiliation country:
Germany