The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS).
BMC Endocr Disord
; 21(1): 119, 2021 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-34130686
ABSTRACT
BACKGROUND:
To explore the development mechanism of PCOS and Transcriptomics was applied to seek the key gene.METHODS:
Transcriptomics marked by UID (unique identifier) technique of granulosa cell in PCOS and control women was carried out and key gene was picked up. Then the key gene in granulosa cell was measured by RT-PCR. Two PCOS models modeling with Letrozole and Testosterone Propionate were implemented and the key gene in granulosa cell of ovary was measured by immunohistochemistry to verify the relation with PCOS.RESULTS:
GO-enrich of transcriptomics concentrated in domain steroid metabolism and domain mitochondria. Different genes were sought from coexisting in both domain steroid metabolism and domain mitochondria. Finally, five different genes including CYP11A1ãCYB5R1ãSTARãFDX1 and AMACR were obtained. RT-PCR was implemented to furtherly verify the downregulating mRNA of FDX1 in PCOS, which showed the consistent outcome with the transcriptomics. Level of FDX1 protein in granulosa cell of antral follicle in two PCOS models was measured and decreased.CONCLUSIONS:
FDX1 was related with steroid metabolism and mitochondrial and may participate in the development of PCOS.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polycystic Ovary Syndrome
/
RNA, Messenger
/
Biomarkers
/
Ferredoxins
/
Granulosa Cells
Type of study:
Observational_studies
/
Prognostic_studies
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
BMC Endocr Disord
Year:
2021
Type:
Article
Affiliation country:
China