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Nano-multilamellar lipid vesicles loaded with a recombinant form of the chikungunya virus E2 protein improve the induction of virus-neutralizing antibodies.
Venceslau-Carvalho, Aléxia Adrianne; Teixeira de Pinho Favaro, Marianna; Ramos Pereira, Lennon; Rodrigues-Jesus, Mônica Josiane; Santos Pereira, Samuel; Andreata-Santos, Robert; Dos Santos Alves, Rúbens Prince; Castro-Amarante, Maria Fernanda; Bitencourt Rodrigues, Karine; Ramos da Silva, Jamile; Rahal Guaragna Machado, Rafael; Dos Passos Cunha, Marielton; Marinho de Andrade Zanotto, Paolo; Luzetti Fotoran, Wesley; Wunderlich, Gerhard; Durigon, Edison Luiz; de Souza Ferreira, Luís Carlos.
Affiliation
  • Venceslau-Carvalho AA; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: alexiabiotec@usp.br.
  • Teixeira de Pinho Favaro M; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: favaro.mtp@gmail.com.
  • Ramos Pereira L; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lennon_rp@hotmail.com.
  • Rodrigues-Jesus MJ; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: modrigues4@gmail.com.
  • Santos Pereira S; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: samuelbiomedicina@usp.br.
  • Andreata-Santos R; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: robert_andreata@hotmail.com.
  • Dos Santos Alves RP; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: rubens.bmc@gmail.com.
  • Castro-Amarante MF; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: mfamarante@gmail.com.
  • Bitencourt Rodrigues K; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: karinebitencourt@usp.br.
  • Ramos da Silva J; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: jamile@usp.br.
  • Rahal Guaragna Machado R; Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: rafaelmachado@usp.br.
  • Dos Passos Cunha M; Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Electronic address: marieltondospassos@gmail.com.
  • Marinho de Andrade Zanotto P; Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Electronic address: pzanotto@usp.br.
  • Luzetti Fotoran W; Unit for Drug Development and Plasmodium Molecular Biology, Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, Brazil. Electronic address: wesleylfw@hotmail.com.
  • Wunderlich G; Unit for Drug Development and Plasmodium Molecular Biology, Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, Brazil. Electronic address: gwunder@usp.br.
  • Durigon EL; Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: eldurigo@usp.br.
  • de Souza Ferreira LC; Vaccine Development Laboratory, Microbiology Department and Parasitology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lcsf@usp.br.
Nanomedicine ; 37: 102445, 2021 10.
Article in En | MEDLINE | ID: mdl-34303841
ABSTRACT
Chikungunya virus (CHIKV) is responsible for a self-limited illness that can evolve into long-lasting painful joint inflammation. In this study, we report a novel experimental CHIKV vaccine formulation of lipid nanoparticles loaded with a recombinant protein derived from the E2 structural protein. This antigen fragment, designated ∆E2.1, maintained the antigenicity of the native viral protein and was specifically recognized by antibodies induced in CHIKV-infected patients. The antigen has been formulated into nanoparticles consisting of nano-multilamellar vesicles (NMVs) combined with the adjuvant monophosphoryl lipid A (MPLA). The vaccine formulation demonstrated a depot effect, leading to controlled antigen release, and induced strong antibody responses significantly higher than in mice immunized with the purified protein combined with the adjuvant. More relevantly, E2-specific antibodies raised in mice immunized with ∆E2.1-loaded NMV-MPLA neutralized CHIKV under in vitro conditions. Taken together, the results demonstrated that the new nanoparticle-based vaccine formulation represents a promising approach for the development of effective anti-CHIKV vaccines.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chikungunya virus / Viral Envelope Proteins / Chikungunya Fever / Liposomes Limits: Animals / Humans Language: En Journal: Nanomedicine Journal subject: BIOTECNOLOGIA Year: 2021 Type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chikungunya virus / Viral Envelope Proteins / Chikungunya Fever / Liposomes Limits: Animals / Humans Language: En Journal: Nanomedicine Journal subject: BIOTECNOLOGIA Year: 2021 Type: Article