The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics.
PLoS Genet
; 17(7): e1009690, 2021 07.
Article
in En
| MEDLINE
| ID: mdl-34319989
ABSTRACT
Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven by endothelial cell (EC) rearrangement, which involves EC nucleus migration, junction remodeling, and actin cytoskeleton remodeling. Further observation reveals a growing difference in blood flow velocity between the two vessels in CV pruning in zebrafish embryos. With this model, we identify the critical role of Kruppel-like factor 6a (klf6a) in CV pruning. Disruption of klf6a functioning impairs CV pruning in zebrafish. klf6a is required for EC nucleus migration, junction remodeling, and actin cytoskeleton dynamics in zebrafish embryos. Moreover, actin-related protein transgelin 2 (tagln2) is a direct downstream target of klf6a in CV pruning in zebrafish embryos. Together these results demonstrate that the klf6a-tagln2 axis regulates CV pruning by promoting EC rearrangement.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Circulation
/
Zebrafish Proteins
/
Microfilament Proteins
/
Muscle Proteins
/
Nerve Tissue Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
PLoS Genet
Journal subject:
GENETICA
Year:
2021
Type:
Article
Affiliation country:
China