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Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis.
Dumas, Sébastien J; Meta, Elda; Conchinha, Nadine V; Sokol, Liliana; Chen, Rongyuan; Borri, Mila; Teuwen, Laure-Anne; Veys, Koen; García-Caballero, Melissa; Geldhof, Vincent; Treps, Lucas; de Zeeuw, Pauline; Falkenberg, Kim D; Dubois, Charlotte; Parys, Magdalena; de Rooij, Laura P M H; Rohlenova, Katerina; Goveia, Jermaine; Schoonjans, Luc; Dewerchin, Mieke; Eelen, Guy; Li, Xuri; Kalucka, Joanna; Carmeliet, Peter.
Affiliation
  • Dumas SJ; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Meta E; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Conchinha NV; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Sokol L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Chen R; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China.
  • Borri M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Teuwen LA; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Veys K; Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium.
  • García-Caballero M; Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium.
  • Geldhof V; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Treps L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • de Zeeuw P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Falkenberg KD; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Dubois C; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Parys M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • de Rooij LPMH; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Rohlenova K; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Goveia J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Schoonjans L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Dewerchin M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Eelen G; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Li X; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China.
  • Kalucka J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
  • Carmeliet P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
STAR Protoc ; 2(3): 100523, 2021 09 17.
Article in En | MEDLINE | ID: mdl-34382011
ABSTRACT
Endothelial cells (ECs) exhibit phenotypic and functional tissue specificities, critical for studies in the vascular field and beyond. Thus, tissue-specific methods for isolation of highly purified ECs are necessary. Kidney, spleen, and testis ECs are relevant players in health and diseases such as chronic kidney disease, acute kidney injury, myelofibrosis, and cancer. Here, we provide tailored protocols for rapid and reproducible EC purification established for scRNA sequencing from these adult murine tissues using the combination of magnetic- and fluorescence-activated cell sorting. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020) and Dumas et al. (2020).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spleen / Testis / Endothelial Cells / Kidney Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Type: Article Affiliation country: Belgium
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spleen / Testis / Endothelial Cells / Kidney Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Type: Article Affiliation country: Belgium