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Simultaneous determination of selected catechins and pyrogallol in deer intoxications by HPLC-MS/MS.
Dutschke, Jennifer; Suchowski, Marcel; Pietsch, Jörg.
Affiliation
  • Dutschke J; University of Applied Sciences Bonn-Rhein-Sieg, von-Liebig-Straße 20, D-53359 Rheinbach, Germany.
  • Suchowski M; Institute of Veterinary Pathology, Universität Leipzig, An den Tierkliniken 33, D-04103 Leipzig, Germany.
  • Pietsch J; Institute of Legal Medicine, Technische Universität Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. Electronic address: Joerg.Pietsch@tu-dresden.de.
Article in En | MEDLINE | ID: mdl-34428648
A selective and sensitive analytical method for the determination of selected catechins (catechin, epicatechin, gallocatechin, epigallocatechin) and pyrogallol in biological matrices by HPLC-MS/MS was developed. The utilized sample preparation technique was a two-stage liquid-liquid extraction using ethyl acetate. The HPLC-system was equipped with a Phenomenex Luna Pentafluorophenyl Column (150 × 2 mm, 5 µm) and operated with an acetonitrile-water gradient as a mobile phase system. Detection was performed with a 3200 Q Trap mass spectrometer. For analysis the mass spectrometer was used in the MRM-mode with negative ionization. The method validation was performed with serum as matrix. The selectivity of the method as well as the linearity of calibration was successfully proven for all analytes. The limits of quantification were between 5.3 and 11.2 ng/mL and the recovery rates were above 50 % for all analytes. Results from the samples of three deer poisoning cases demonstrated that the developed HPLC-MS/MS method is applicable to real biological samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Deer / Catechin Type of study: Prognostic_studies Limits: Animals Country/Region as subject: Europa Language: En Journal: J Chromatogr B Analyt Technol Biomed Life Sci Journal subject: ENGENHARIA BIOMEDICA Year: 2021 Type: Article Affiliation country: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Deer / Catechin Type of study: Prognostic_studies Limits: Animals Country/Region as subject: Europa Language: En Journal: J Chromatogr B Analyt Technol Biomed Life Sci Journal subject: ENGENHARIA BIOMEDICA Year: 2021 Type: Article Affiliation country: Germany