Simulating cellular galectin networks by mixing galectins in vitro reveals synergistic activity.
Biochem Biophys Rep
; 28: 101116, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34485713
ABSTRACT
BACKGROUND:
Even though members of the family of adhesion/growth-regulatory galectins are increasingly detected to be co-expressed, they are still being routinely tested separately. The recent discovery of heterodimer formation among galectins-1, -3, and -7 in mixtures prompts further study of their functional activities in mixtures.METHODS:
Cell agglutination, galectin binding to cells, as well as effects on cell proliferation, onset of apoptosis and migration were determined in assays using various cell types and mixtures of galectins-1, -3, and -7.RESULTS:
Evidence for a more than additive increases of experimental parameters was consistently obtained.CONCLUSION:
Testing galectins in mixtures simulates the situation of co-expression in situ and reveals unsuspected over-additive activities. This new insight is relevant for analyzing galectin functionality in (patho)physiological conditions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Biochem Biophys Rep
Year:
2021
Type:
Article
Affiliation country:
United States