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In vivo imaging of the inner retinal layer structure in mice after eye-opening using visible-light optical coherence tomography.
Beckmann, Lisa; Cai, Zhen; Cole, James; Miller, David A; Liu, Mingna; Grannonico, Marta; Zhang, Xian; Ryu, Hyun Jung; Netland, Peter A; Liu, Xiaorong; Zhang, Hao F.
Affiliation
  • Beckmann L; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Cai Z; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Cole J; Department of Biology, University of Virginia, Charlottesville, VA, USA.
  • Miller DA; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Liu M; Department of Biology, University of Virginia, Charlottesville, VA, USA.
  • Grannonico M; Department of Biology, University of Virginia, Charlottesville, VA, USA.
  • Zhang X; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Ryu HJ; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Netland PA; Department of Ophthalmology, University of Virginia, Charlottesville, VA, USA.
  • Liu X; Department of Biology, University of Virginia, Charlottesville, VA, USA; Department of Ophthalmology, University of Virginia, Charlottesville, VA, USA; Department of Psychology, University of Virginia, Charlottesville, VA, USA. Electronic address: xl8n@virginia.edu.
  • Zhang HF; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA. Electronic address: hfzhang@northwestern.edu.
Exp Eye Res ; 211: 108756, 2021 10.
Article in En | MEDLINE | ID: mdl-34492282
ABSTRACT
The growth of the mouse eye and retina after birth is a dynamic, highly regulated process. In this study, we applied visible-light optical coherence tomography (vis-OCT), a non-invasive imaging technique, to examine developing retinal layer structures after eye-opening. We introduced a resampled circumpapillary B-scan averaging technique to improve the inter-layer contrast, enabling retinal layer thickness measurements as early as postnatal day 13 (P13) - right after eye-opening. We confirmed vis-OCT measurements using ex vivo confocal microscopy of retinal sections at different ages. Our results demonstrate that vis-OCT can visualize the developmental murine retinal layer structure in vivo, which offers us new opportunities to better characterize the pathological alterations in mouse models of developmental eye diseases.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retina Limits: Animals Language: En Journal: Exp Eye Res Year: 2021 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retina Limits: Animals Language: En Journal: Exp Eye Res Year: 2021 Type: Article Affiliation country: United States