Generation of 'designer erythroblasts' lacking one or more blood group systems from CRISPR/Cas9 gene-edited human-induced pluripotent stem cells.

Pandey, Priyanka; Zhang, Nanyan; Curtis, Brian R; Newman, Peter J; Denomme, Gregory A

Pandey, Priyanka; Zhang, Nanyan; Curtis, Brian R; Newman, Peter J; Denomme, Gregory A.

Affiliation

Pandey P; Versiti Blood Research Institute, Milwaukee, WI, USA.
Zhang N; Versiti Blood Research Institute, Milwaukee, WI, USA.
Curtis BR; Versiti Blood Research Institute, Milwaukee, WI, USA.
Newman PJ; Diagnostic Laboratories, Versiti Blood Center of Wisconsin, Milwaukee, WI, USA.
Denomme GA; Versiti Blood Research Institute, Milwaukee, WI, USA.

J Cell Mol Med ; 25(19): 9340-9349, 2021 10.

Article in En | MEDLINE | ID: mdl-34547166

Subject(s)

CRISPR-Cas Systems; Cell Differentiation; Cell Lineage; Erythroblasts/metabolism; Gene Editing; Induced Pluripotent Stem Cells/metabolism; Biomarkers; Blood Group Antigens/genetics; Blood Group Antigens/metabolism; Cell Line; Erythroblasts/cytology; Gene Knockdown Techniques; Hematopoiesis; Histocytochemistry; Humans; Immunophenotyping; Induced Pluripotent Stem Cells/cytology; RNA, Guide, Kinetoplastida/genetics

Key words

CRISPR; alloimmunization; antibody identification; blood group systems; erythroblasts; hiPSC

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / Erythroblasts / Cell Lineage / Induced Pluripotent Stem Cells / CRISPR-Cas Systems / Gene Editing Limits: Humans Language: En Journal: J Cell Mol Med Journal subject: BIOLOGIA MOLECULAR Year: 2021 Type: Article Affiliation country: United States

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