Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans.

ABSTRACT

To generate a non-red/green fluorescent fusion histone protein in C. elegans, we have generated a C-terminal mTurquoise2-tagged HIS-72 at the endogenous locus using CRISPR. We found that HIS-72mTurquoise2 localizes in a similar pattern to the previously published HIS-72GFP strain.