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Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.
Bhandari, Bikash K; Lim, Chun Shen; Remus, Daniela M; Chen, Augustine; van Dolleweerd, Craig; Gardner, Paul P.
Affiliation
  • Bhandari BK; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
  • Lim CS; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
  • Remus DM; Callaghan Innovation Protein Science and Engineering, University of Canterbury, Christchurch, New Zealand.
  • Chen A; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
  • van Dolleweerd C; Biomolecular Interaction Center, University of Canterbury, Christchurch, New Zealand.
  • Gardner PP; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
PLoS Comput Biol ; 17(10): e1009461, 2021 10.
Article in En | MEDLINE | ID: mdl-34610008
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Codon, Terminator / Codon, Initiator / Silent Mutation Type of study: Prognostic_studies Language: En Journal: PLoS Comput Biol Journal subject: BIOLOGIA / INFORMATICA MEDICA Year: 2021 Type: Article Affiliation country: New Zealand
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Codon, Terminator / Codon, Initiator / Silent Mutation Type of study: Prognostic_studies Language: En Journal: PLoS Comput Biol Journal subject: BIOLOGIA / INFORMATICA MEDICA Year: 2021 Type: Article Affiliation country: New Zealand