Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells.

Long, Kimberly R; Rbaibi, Youssef; Bondi, Corry D; Ford, B Rhodes; Poholek, Amanda C; Boyd-Shiwarski, Cary R; Tan, Roderick J; Locker, Joseph D; Weisz, Ora A

Long, Kimberly R; Rbaibi, Youssef; Bondi, Corry D; Ford, B Rhodes; Poholek, Amanda C; Boyd-Shiwarski, Cary R; Tan, Roderick J; Locker, Joseph D; Weisz, Ora A.

Affiliation

Long KR; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Rbaibi Y; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Bondi CD; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Ford BR; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Poholek AC; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Boyd-Shiwarski CR; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Tan RJ; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Locker JD; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Weisz OA; Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.

Am J Physiol Renal Physiol ; 322(1): F14-F26, 2022 01 01.

Article in En | MEDLINE | ID: mdl-34747197

Subject(s)

Adaptor Proteins, Signal Transducing/metabolism; Apoptosis Regulatory Proteins/metabolism; CRISPR-Associated Protein 9/genetics; CRISPR-Cas Systems; Gene Knockout Techniques; Kidney Tubules, Proximal/metabolism; Low Density Lipoprotein Receptor-Related Protein-2/metabolism; Receptors, Cell Surface/metabolism; Transcription, Genetic; Adaptor Proteins, Signal Transducing/genetics; Agenesis of Corpus Callosum/genetics; Agenesis of Corpus Callosum/metabolism; Agenesis of Corpus Callosum/pathology; Animals; Apoptosis Regulatory Proteins/genetics; Cells, Cultured; Databases, Genetic; Gene Regulatory Networks; Hearing Loss, Sensorineural/genetics; Hearing Loss, Sensorineural/metabolism; Hearing Loss, Sensorineural/pathology; Hernias, Diaphragmatic, Congenital/genetics; Hernias, Diaphragmatic, Congenital/metabolism; Hernias, Diaphragmatic, Congenital/pathology; Humans; Kidney Tubules, Proximal/pathology; Low Density Lipoprotein Receptor-Related Protein-2/genetics; Male; Mice, Knockout; Monodelphis; Myopia/genetics; Myopia/metabolism; Myopia/pathology; Proteinuria/genetics; Proteinuria/metabolism; Proteinuria/pathology; Receptors, Cell Surface/genetics; Renal Tubular Transport, Inborn Errors/genetics; Renal Tubular Transport, Inborn Errors/metabolism; Renal Tubular Transport, Inborn Errors/pathology

Key words

Donnai-Barrow syndrome; endocytosis; renal proximal tubule cell; sodium-glucose cotransporter isoform 2; transcriptional profiling

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription, Genetic / Receptors, Cell Surface / Low Density Lipoprotein Receptor-Related Protein-2 / Adaptor Proteins, Signal Transducing / Apoptosis Regulatory Proteins / Gene Knockout Techniques / CRISPR-Cas Systems / CRISPR-Associated Protein 9 / Kidney Tubules, Proximal Type of study: Prognostic_studies Language: En Journal: Am J Physiol Renal Physiol Journal subject: FISIOLOGIA / NEFROLOGIA Year: 2022 Type: Article

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