Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata.
BMC Plant Biol
; 21(1): 559, 2021 Nov 25.
Article
in En
| MEDLINE
| ID: mdl-34823468
ABSTRACT
BACKGROUND:
Sucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of the INV gene family in Poncirus trifoliata or its Citrus relatives in response to abiotic stresses are not fully understood.RESULTS:
In this report, fourteen non-redundant PtrINV family members were identified in P. trifoliata including seven alkaline/neutral INV genes (PtrA/NINV1-7), two vacuolar INV genes (PtrVINV1-2), and five cell wall INV isoforms (PtrCWINV1-5). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon-intron structures and the evolutionary relationships demonstrated the conservation and the divergence of PtrINVs. In addition, expression analysis of INV genes during several abiotic stresses in various tissues indicated the central role of A/NINV7 among INV family members in response to abiotic stresses. Furthermore, our data demonstrated that high accumulation of Suc, Glc, Frc and total sugar contents were directly correlated with the elevated activities of soluble INV enzymes in the cold-tolerant P. trifoliata, C. ichangensis and C. sinensis, demonstrating the potential role of soluble INV enzymes for the cold tolerance of Citrus.CONCLUSIONS:
This work offered a framework for understanding the physiological role of INV genes and laid a foundation for future functional studies of these genes in response to abiotic stresses.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sucrose
/
Adaptation, Physiological
/
Citrus
/
Cold Temperature
/
Poncirus
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
BMC Plant Biol
Journal subject:
BOTANICA
Year:
2021
Type:
Article
Affiliation country:
China