A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker.
Sensors (Basel)
; 21(22)2021 Nov 11.
Article
in En
| MEDLINE
| ID: mdl-34833562
ABSTRACT
Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a "turn-on" bioluminescent readout of a C. difficile-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was "caged" with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clostridioides difficile
/
Clostridioides
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Sensors (Basel)
Year:
2021
Type:
Article
Affiliation country:
United States