Your browser doesn't support javascript.
loading
Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
Michalik, Stephan; Siegerist, Florian; Palankar, Raghavendra; Franzke, Kati; Schindler, Maximilian; Reder, Alexander; Seifert, Ulrike; Cammann, Clemens; Wesche, Jan; Steil, Leif; Hentschker, Christian; Gesell-Salazar, Manuela; Reisinger, Emil; Beer, Martin; Endlich, Nicole; Greinacher, Andreas; Völker, Uwe.
Affiliation
  • Michalik S; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
  • Siegerist F; Institute for Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.
  • Palankar R; Institute of Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany.
  • Franzke K; Institute of Infectiology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
  • Schindler M; Institute for Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.
  • Reder A; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
  • Seifert U; Friedrich Loeffler-Institute of Medical Microbiology-Virology, University Medicine Greifswald, Greifswald, Germany..
  • Cammann C; Friedrich Loeffler-Institute of Medical Microbiology-Virology, University Medicine Greifswald, Greifswald, Germany..
  • Wesche J; Institute of Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany.
  • Steil L; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
  • Hentschker C; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
  • Gesell-Salazar M; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
  • Reisinger E; Division of Tropical Medicine and Infectious Diseases, Center of Internal Medicine II, Rostock University Medical Center, Rostock, Germany.
  • Beer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
  • Endlich N; Institute for Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.
  • Greinacher A; Institute of Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany. andreas.greinacher@med.unigreifswald.
  • Völker U; Interfaculty Institute of Genetics and Functional Genomics, Department Functional Genomics, University Medicine Greifswald, Greifswald, Germany. voelker@uni-greifswald.de.
Haematologica ; 107(4): 947-957, 2022 04 01.
Article in En | MEDLINE | ID: mdl-35045692
Vector-based SARS-CoV-2 vaccines have been associated with vaccine- induced thrombosis with thrombocytopenia syndrome (VITT/TTS), but the causative factors are still unresolved. We comprehensively analyzed the ChAdOx1 nCoV-19 (AstraZeneca) and Ad26.COV2.S (Johnson and Johnson) vaccines. ChAdOx1 nCoV-19 contains significant amounts of host cell protein impurities, including functionally active proteasomes, and adenoviral proteins. A much smaller amount of impurities was found in Ad26.COV2.S. Platelet factor 4 formed complexes with ChAdOx1 nCoV-19 constituents, but not with purified virions from ChAdOx1 nCoV-19 or with Ad26.COV2.S. Vascular hyperpermeability was induced by ChAdOx nCoV-19 but not by Ad26.COV2.S. These differences in impurities together with EDTAinduced capillary leakage might contribute to the higher incidence rate of VITT associated with ChAdOx1 nCoV-19 compared to Ad26.COV2.S.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vaccines / COVID-19 Limits: Humans Language: En Journal: Haematologica Year: 2022 Type: Article Affiliation country: Germany
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vaccines / COVID-19 Limits: Humans Language: En Journal: Haematologica Year: 2022 Type: Article Affiliation country: Germany