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Optimization of VSV-ΔG-spike production process with the Ambr15 system for a SARS-COV-2 vaccine.
Rosen, Osnat; Jayson, Avital; Goldvaser, Michael; Dor, Eyal; Monash, Arik; Levin, Lilach; Cherry, Lilach; Lupu, Edith; Natan, Niva; Girshengorn, Meni; Epstein, Eyal.
Affiliation
  • Rosen O; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Jayson A; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Goldvaser M; Department of Organic Chemistry, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Dor E; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Monash A; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Levin L; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Cherry L; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Lupu E; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Natan N; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Girshengorn M; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
  • Epstein E; Department of Biotechnology, Israel Institute for Biological, Chemical and Environmental Sciences, Ness Ziona, Israel.
Biotechnol Bioeng ; 119(7): 1839-1848, 2022 07.
Article in En | MEDLINE | ID: mdl-35319097
ABSTRACT
To face the coronavirus disease 2019 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, our institute has developed the rVSV-ΔG-spike vaccine, in which the glycoprotein of vesicular stomatitis virus (VSV) was replaced by the spike protein of SARS-CoV-2. Many process parameters can influence production yield. To maximize virus vaccine yield, each parameter should be tested independently and in combination with others. Here, we report the optimization of the production of the VSV-ΔG-spike vaccine in Vero cells using the Ambr15 system. This system facilitates high-throughput screening of process parameters, as it contains 24 individually controlled, single-use stirred-tank minireactors. During optimization, critical parameters were tested. Those parameters included cell densities; the multiplicity of infection; virus production temperature; medium addition and medium exchange; and supplementation of glucose in the virus production step. Virus production temperature, medium addition, and medium exchange were all found to significantly influence the yield. The optimized parameters were tested in the BioBLU 5p bioreactors production process and those that were found to contribute to the vaccine yield were integrated into the final process. The findings of this study demonstrate that an Ambr15 system is an effective tool for bioprocess optimization of vaccine production using macrocarriers and that the combination of production temperature, rate of medium addition, and medium exchange significantly improved virus yield.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: COVID-19 Vaccines / COVID-19 Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2022 Type: Article Affiliation country: Israel

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: COVID-19 Vaccines / COVID-19 Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2022 Type: Article Affiliation country: Israel