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Potent neutralization by monoclonal human IgM against SARS-CoV-2 is impaired by class switch.
Callegari, Ilaria; Schneider, Mika; Berloffa, Giuliano; Mühlethaler, Tobias; Holdermann, Sebastian; Galli, Edoardo; Roloff, Tim; Boss, Renate; Infanti, Laura; Khanna, Nina; Egli, Adrian; Buser, Andreas; Zimmer, Gert; Derfuss, Tobias; Sanderson, Nicholas S R.
Affiliation
  • Callegari I; Department of Biomedicine, University of Basel and University Hospital Basel, Basel, Switzerland.
  • Schneider M; MS Center, Neurologic Clinic and Policlinic, Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University Hospital Basel, University of Basel, Basel, Switzerland.
  • Berloffa G; Department of Biomedicine, University of Basel and University Hospital Basel, Basel, Switzerland.
  • Mühlethaler T; Department of Biomedicine, University of Basel and University Hospital Basel, Basel, Switzerland.
  • Holdermann S; Biophysics Facility, Biozentrum, University of Basel, Basel, Switzerland.
  • Galli E; Department of Biomedicine, University of Basel and University Hospital Basel, Basel, Switzerland.
  • Roloff T; MS Center, Neurologic Clinic and Policlinic, Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University Hospital Basel, University of Basel, Basel, Switzerland.
  • Boss R; Department of Biomedicine, University of Basel and University Hospital Basel, Basel, Switzerland.
  • Infanti L; MS Center, Neurologic Clinic and Policlinic, Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University Hospital Basel, University of Basel, Basel, Switzerland.
  • Khanna N; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
  • Egli A; Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland.
  • Buser A; Federal Food Safety and Veterinary Office, Bern, Switzerland.
  • Zimmer G; Regional Blood Transfusion Service, Swiss Red Cross, Basel, Switzerland.
  • Derfuss T; Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
  • Sanderson NSR; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
EMBO Rep ; 23(7): e53956, 2022 07 05.
Article in En | MEDLINE | ID: mdl-35548920
ABSTRACT
To investigate the class-dependent properties of anti-viral IgM antibodies, we use membrane antigen capture activated cell sorting to isolate spike-protein-specific B cells from donors recently infected with SARS-CoV-2, allowing production of recombinant antibodies. We isolate 20, spike-protein-specific antibodies of classes IgM, IgG, and IgA, none of which shows any antigen-independent binding to human cells. Two antibodies of class IgM mediate virus neutralization at picomolar concentrations, but this potency is lost following artificial switch to IgG. Although, as expected, the IgG versions of the antibodies appear to have lower avidity than their IgM parents, this is not sufficient to explain the loss of potency.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Humans Language: En Journal: EMBO Rep Journal subject: BIOLOGIA MOLECULAR Year: 2022 Type: Article Affiliation country: Switzerland
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Humans Language: En Journal: EMBO Rep Journal subject: BIOLOGIA MOLECULAR Year: 2022 Type: Article Affiliation country: Switzerland