Innovative Approach in the Cryogenic Freezing Medium for Mesenchymal Stem Cells.
Biomolecules
; 12(5)2022 04 20.
Article
in En
| MEDLINE
| ID: mdl-35625538
ABSTRACT
The physical stresses during cryopreservation affect stem cell survival and further proliferation. To minimize or prevent cryoinjury, cryoprotective agents (CPAs) are indispensable. Despite the widespread use of 10% dimethyl sulfoxide (DMSO), there are concerns about its potential adverse effects. To bypass those effects, combinations of CPAs have been investigated. This study aimed to verify whether high-molecular-hyaluronic acid (HMW-HA) serves as a cryoprotectant when preserving human mesenchymal stem cells (hMSCs) to reduce the DMSO concentration in the cryopreservation medium. We studied how 0.1% or 0.2% HMW-HA combined with reduced DMSO concentrations (from 10% to 5%, and 3%) affected total cell count, viability, immunophenotype, and differentiation potential post-cryopreservation. Immediately after cell revival, the highest total cell count was observed in 10% DMSO-stored hMSC. However, two weeks after cell cultivation an increased cell count was seen in the HMW-HA-stored groups along with a continued increase in hMSCs stored using 3% DMSO and 0.1% HMW-HA. The increased total cell count corresponded to elevated expression of stemness marker CD49f. The HA-supplemented cryomedium did not affect the differential potential of hMSC. Our results will participate in producing a ready-to-use product for cryopreservation of mesenchymal stem cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dimethyl Sulfoxide
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Biomolecules
Year:
2022
Type:
Article
Affiliation country:
Czech Republic