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Fingerprinting of skin cells by live cell Raman spectroscopy reveals melanoma cell heterogeneity and cell-type-specific responses to UVR.
Wilkinson, Emma L; Ashton, Lorna; Kerns, Jemma G; Allinson, Sarah L; Mort, Richard L.
Affiliation
  • Wilkinson EL; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
  • Ashton L; Department of Chemistry, Lancaster University, Lancaster, UK.
  • Kerns JG; Lancaster Medical School, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
  • Allinson SL; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
  • Mort RL; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
Exp Dermatol ; 31(10): 1543-1553, 2022 10.
Article in En | MEDLINE | ID: mdl-35700136
ABSTRACT
Raman spectroscopy is an emerging dermatological technique with the potential to discriminate biochemically between cell types in a label-free and non-invasive manner. Here, we use live single-cell Raman spectroscopy and principal component analysis (PCA) to fingerprint mouse melanoblasts, melanocytes, keratinocytes and melanoma cells. We show the differences in their spectra are attributable to biomarkers in the melanin biosynthesis pathway and that melanoma cells are a heterogeneous population that sit on a trajectory between undifferentiated melanoblasts and differentiated melanocytes. We demonstrate the utility of Raman spectroscopy as a highly sensitive tool to probe the melanin biosynthesis pathway and its immediate response to ultraviolet (UV) irradiation revealing previously undescribed opposing responses to UVA and UVB irradiation in melanocytes. Finally, we identify melanocyte-specific accumulation of ß-carotene correlated with a stabilisation of the UVR response in lipids and proteins consistent with a ß-carotene-mediated photoprotective mechanism. In summary, our data show that Raman spectroscopy can be used to determine the differentiation status of cells of the melanocyte lineage and describe the immediate and temporal biochemical changes associated with UV exposure which differ depending on cell type, differentiation status and competence to synthesise melanin. Our work uniquely applies Raman spectroscopy to discriminate between cell types by biological function and differentiation status while they are growing in culture. In doing so, we demonstrate for the first time its utility as a tool with which to probe the melanin biosynthesis pathway.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Melanins / Melanoma Limits: Animals Language: En Journal: Exp Dermatol Journal subject: DERMATOLOGIA Year: 2022 Type: Article Affiliation country: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Melanins / Melanoma Limits: Animals Language: En Journal: Exp Dermatol Journal subject: DERMATOLOGIA Year: 2022 Type: Article Affiliation country: United kingdom