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Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion.
Mechrez, Guy; Mani, Karthik Ananth; Saha, Abhijit; Lachman, Oded; Luria, Neta; Molad, Ori; Kotliarevski, Liliya; Zelinger, Einat; Smith, Elisheva; Yaakov, Noga; Stone, Dalia Shabashov; Reches, Meital; Dombrovsky, Aviv.
Affiliation
  • Mechrez G; Department of Food Science, Institute of Postharvest and Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Mani KA; Department of Food Science, Institute of Postharvest and Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Saha A; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
  • Lachman O; Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
  • Luria N; Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203, India.
  • Molad O; Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Kotliarevski L; Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Zelinger E; Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
  • Smith E; Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Yaakov N; Department of Food Science, Institute of Postharvest and Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
  • Stone DS; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
  • Reches M; The Interdepartmental Equipment Unit, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
  • Dombrovsky A; Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
ACS Appl Bio Mater ; 5(8): 3859-3869, 2022 08 15.
Article in En | MEDLINE | ID: mdl-35913405
The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers. The resultant emulsions were stable for weeks in ambient conditions, and our platform was challenged using the epitope of the SARS-CoV-2-S1 peptide that served as a model epitope in this study. In vivo assays showed that the αSARS-CoV-2-S1 immunoglobulin G (IgG) titers of the studied mouse antisera, developed against the SARS-CoV-2-S1 peptide under different epitope preparation conditions, showed an order of magnitude higher IgG titers in the studied VLP-based emulsions than epitopes dissolved in water and epitopes administered with an adjuvant, thereby confirming the efficacy of the formulation. This VLP-based Pickering emulsion platform is a fully synthetic approach that can be readily applied for vaccine development to a wide range of pathogens.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Animals Language: En Journal: ACS Appl Bio Mater Year: 2022 Type: Article Affiliation country: Israel
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Animals Language: En Journal: ACS Appl Bio Mater Year: 2022 Type: Article Affiliation country: Israel